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PRDX3 Recombinant Monoclonal Antibody

  • 中文名稱：
    PRDX3重組抗體
  • 貨號：
    CSB-RA437344A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: PRDX3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28, 26 kDa
      Observed band size: 20-28 kDa
    • IHC image of CSB-RA437344A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA437344A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA437344A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 503-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA437344A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA437344A0HU PRDX3重組單克隆抗體是針對過氧化物酶3（PRDX3）研發的高特異性試劑，適用于多種蛋白檢測實驗。該抗體采用重組技術制備，可特異性識別PRDX3蛋白，其廣泛驗證的實驗應用包括ELISA、蛋白印跡（推薦稀釋1:500-1:2000）、免疫組化（1:50-1:200）、免疫熒光（1:50-1:200）及流式細胞術（1:50-1:200）。實驗數據顯示，該抗體在多種樣本類型中均展現出優異的特異性與靈敏度，能清晰識別目標蛋白表達定位，尤其適用于線粒體相關氧化應激研究。作為線粒體抗氧化系統的核心成員，PRDX3在細胞氧化還原調控、凋亡及代謝相關通路研究中具有重要價值。本產品可為神經退行性疾病、腫瘤發生機制及氧化損傷修復等基礎研究提供可靠工具，支持線粒體功能分析、氧化應激模型驗證等實驗場景。嚴格的質量控制確保批次間一致性，所有實驗數據均經過多模型驗證，滿足科研領域對數據可重復性的高標準要求。本產品僅限科學研究使用。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Thioredoxin-dependent peroxide reductase, mitochondrial (EC 1.11.1.15) (Antioxidant protein 1) (AOP-1) (HBC189) (Peroxiredoxin III) (Prx-III) (Peroxiredoxin-3) (Protein MER5 homolog), PRDX3, AOP1
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PRDX3
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    14E2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
  • 基因功能參考文獻：
    1. tubular PRX3 in combination with conventional predictors can further improve recovery prediction and may help with risk stratification in AKI patients with pre-existing CKD. PMID: 28240739
    2. results suggest that decreased PRDX3 by excessive bile acids in trophoblasts plays a critical role in the pathogenesis and progression of Intrahepatic cholestasis of pregnancy. PMID: 27958341
    3. Mutants created to investigate link between oligomerisation and peroxidase activity. Obligate dimer is less active than wildtype but still displays peroxidase activity. 2.4A structure (PDB: 5UCX) shows cysteine-stabilised protein interface. PMID: 29438714
    4. the present study identified the protein profiles of the PRDX family in LSCC and showed that down-regulation of PRDX3 in Hep-2 cells induced cell apoptosis and inhibited cell proliferation and cell migration. PMID: 27966448
    5. The UNG1-PRDX3 interaction protected UNG1 from reactive oxygen species-mediated degradation and prevented mitochondrial DNA oxidation. PMID: 27480846
    6. PRDX3 knockdown led to the down-regulation of ATP synthases and the decreased cellular ATP level, contributing to the slow-down of cell growth. Furthermore, silencing PRDX3 enhanced invasive properties of HepG2 cells via TIMP-1 down-regulation and the increased ECM degradation PMID: 26983019
    7. Prx3 and Trx2 comprise an adaptive system to sense changes in atmospheric oxygen tension and influence cellular injury responses through both detoxification of mitochondrial oxidants and regulation of mitochondrial redox-dependent signaling PMID: 28045936
    8. PRX3 plays a crucial role in mitochondrial homeostasis. PMID: 27037278
    9. High PRX3 expression is associated with invasion of lung adenocarcinoma. PMID: 26810067
    10. We also found that forkhead box protein 1 (FOXM1)-induced peroxiredoxin 3 (PRDX3) maintains the mitochondrial function, and the FOXM1/PRDX3 mitochondrial pathway maintains survival of colon CSCs PMID: 26350748
    11. Oxidative stress promotes PRX2 and PRX3 hyperoxidation and attenuates pro-survival signaling in aging chondrocytes. PMID: 26797130
    12. the species with one disulfide and one hyperoxidized active site was decameric for Prx2 and dimeric for Prx3. Reduction and re-oxidation of the hyperoxidized dimer of Prx3 produced hyperoxidized monomers PMID: 26614766
    13. Data indicate that peroxiredoxin III (PrxIII) is a common direct target of both miroRNAs miR-26a-5p and miR-23b-3p. PMID: 24828865
    14. Colon cancer stem cells overexpress the mitochondrial gene PRX3, which is required for maintenance of mitochondrial function and tumorigenesis, and is regulated by forkhead box protein 1, which also regulates expression of CD133 in these cells. PMID: 26091938
    15. High serum PRDX3 levels are associated with hepatocellular carcinoma. PMID: 25743848
    16. Peroxiredoxin 3 levels regulate a mitochondrial redox setpoint in malignant mesothelioma cells. PMID: 25462069
    17. two placental proteins, Prx3 and Prx4, may act as new placental immune targets. PMID: 25323516
    18. Serum PRDX3 can be used as a noninvasive biomarker for the diagnosis and/or prognosis of hepatocellular carcinoma. PMID: 24815434
    19. High peroxiredoxin 3 protein expression is associated with hepatocellular carcinoma. PMID: 24105047
    20. HIF-1alpha is a transcription factor for PRDX3 gene expression in clear cell renal cell carcinoma. PMID: 25093297
    21. Data indicate that engineered Prx2 and Prx3 variants C-terminal residues modulate the extent of hyperoxidation. PMID: 24003226
    22. The silencing of PRDX3 triggered cisplatinmediated apoptosis in the ovarian cancer cells, which may act through suppression of the NFkappaB signaling pathway. PMID: 23503975
    23. Results suggest that PRDX-3 has an essential role in regulating oxidation-induced apoptosis in antiandrogen-resistant prostate cancer cells. PMID: 23880827
    24. peroxiredoxin III may be associated with drug resistance in ovarian cancer PMID: 23564483
    25. A fraction of FOXM1 coexists in the cytoplasm with mitochondrial peroxiredoxin 3. PMID: 23018647
    26. Drug resistance formation was accompanied by a significant increase in the expression of PRDX1, PRDX2, PRDX3, PRDX6 genes in all cancer cell strains, which confirms the importance of redox-dependent mechanisms into development of cisplatin resistance. PMID: 23113308
    27. Medulloblastoma tumors exhibit decreased expression of miR-383 but elevated expression of PRDX3. Up-regulation of miR-383 knocks down the expression of PRDX3, inhibits cell proliferation and promotes apoptosis. PMID: 23157748
    28. the effects of PRDX3 in PCa progression may be caused by the regulation function of miR-23b, and consequently, miR-23b may be involved in the response of PCa cells to hypoxia stress PMID: 22710126
    29. Single nucleotide polymorphisms in the PRDX3 and RPS19 is associated with HPV persistence and cervical precancer/cancer. PMID: 22496757
    30. The results indicated that overexpression of PRDX3 was associated with 94.9% hepatocellular carcinoma, and correlated with poor differentiation (P<0.05), which suggest that PRDX3 has substantial clinical impact on the progression of hepatocarcinoma. PMID: 22344546
    31. Report nuclear factor E2-related factor 2 dependent overexpression of sulfiredoxin and peroxiredoxin III in human lung cancer. PMID: 22016591
    32. Cullin 4B protein ubiquitin ligase targets peroxiredoxin III for degradation. PMID: 21795677
    33. The interaction between genetic variations in the PRDX3 gene and dietary fat intake is important for modulation of BMI and obesity risk. PMID: 21127481
    34. Model for the exceptional reactivity of peroxiredoxins 2 and 3 with hydrogen peroxide: a kinetic and computational study. PMID: 21385867
    35. Paraneoplastic autoimmune reaction against retinal pigment epithelium, with PRDX3 as the putative antigen, may be a cause of acute exudative polymorphous vitelliform maculopathy (AEPVM). PMID: 21220625
    36. Both thioredoxin 2 and glutaredoxin 2 contribute to the reduction of the mitochondrial 2-Cys peroxiredoxin Prx3. PMID: 20929858
    37. Prx3, which resides within mitochondria, were reacted with a selection of hydroperoxides generated by gamma-radiolysis or singlet oxygen, on free amino acids, peptides and proteins. PMID: 20840079
    38. Peroxiredoxin III was significantly up-regulated in cervical cancer tissues. PMID: 20718351
    39. Peroxiredoxin III has a significant role in cell cycle regulation and could be a potential proliferation marker in breast cancer. PMID: 20043069
    40. Protein levels of Prx-III, a mitochondrial protein were significantly decreased in Alzheimer Disease and Down Syndrome. PMID: 11771746
    41. Proteomics analysis of cellular response to oxidative stress. Evidence for in vivo overoxidation of peroxiredoxins at their active site PMID: 11904290
    42. PRDX3 is a c-Myc target gene that is requried to maintain normal mitochondrial function. c-Myc directly activates expression of a mitochondrial peroxiredoxin that is required for Myc-mediated transformation. PMID: 12011429
    43. LZK was associated directly with the IKK complex through the kinase domain, and that AOP-1 was recruited to the IKK complex through the binding to LZK. PMID: 12492477
    44. The level of peroxiredoxin 3 is decreased in the frontal cortex of patients with Down syndrome and Pick's disease. PMID: 12650976
    45. Peroxiredoxin III is a critical regulator of the abundance of mitochondrial H(2)O(2), which itself promotes apoptosis in cooperation with other mediators of apoptotic signaling PMID: 15280382
    46. findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking. PMID: 15750338
    47. The additive protection by Trx2 and GSH shows that Trx2 and GSH systems are both functionally important at low oxidative stress conditions. PMID: 17548047
    48. Finally, the expression level of beta-globin and GATA-1 was dramatically increased in Prdx III O/E cell line. PMID: 17574212
    49. Present throughout lens and localized to mitochondria in lens epithelial cells. Induction is acute response of lens to increased H2O2. Important role in lens H2O2-detoxification, mitochondrial maintenance, and possibly cataract formation. PMID: 17893648
    50. FOXO3A mediates Prx III expression, and this may play a critical role in the resistance to oxidative stress in cardiac fibroblasts PMID: 18195003

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  • 亞細胞定位：
    Mitochondrion. Cytoplasm. Early endosome.
  • 蛋白家族：
    Peroxiredoxin family, AhpC/Prx1 subfamily
  • 數據庫鏈接：

    HGNC: 9354

    OMIM: 604769

    KEGG: hsa:10935

    STRING: 9606.ENSP00000298510

    UniGene: Hs.523302



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