LEMD1 Antibody

中文名稱：

LEMD1兔多克隆抗體
貨號：

CSB-PA715035LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA715035LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA715035LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) LEMD1 Polyclonal antibody
Uniprot No.：

Q68G75
基因名：

LEMD1
別名：

LEMD1; LEM domain-containing protein 1; Cancer/testis antigen 50; CT50; LEM domain protein 1; LEMP-1
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human LEM domain-containing protein 1 protein (44-151AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，LEMD1 Antibody (CSB-PA715035LA01HU)，的標記方式是Non-conjugated。對于LEMD1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA715035LB01HU	LEMD1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA715035LC01HU	LEMD1 Antibody, FITC conjugated
Biotin	CSB-PA715035LD01HU	LEMD1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

基因功能參考文獻：
1. Data show that LEM domain containing 1 (LEMD1)has five transcript splicing variants (variant 1 [V1] - variant 5 [V5]), and that LEMD1 V1, V2 and V3 is expressed in testis and CR-CSCs/CICs, whereas LEMD1 V4 and V5 is ubiquitously expressed. PMID: 28219643
2. Downregulation of LEMD1 expression inhibited adhesion and transmigration of OSCCs. PMID: 27280633
3. Of the prostate cancer samples, 10, 10, 23 and 40% showed ODF1, ODF2, LEMD1 and SPATA19 specific bands, respectively, but none of the BPH samples expressed any of these genes. PMID: 20682177
4. LEM domain-containing 1 is a novel testis-specific gene expressed in colorectal cancers PMID: 15254688
亞細胞定位：

Membrane; Single-pass membrane protein.
組織特異性：

Testis-specific. Isoform 6 is detected in 17 of 18 colon cancer tissues examined.
數據庫鏈接：

HGNC: 18725

OMIM: 610480

KEGG: hsa:93273

STRING: 9606.ENSP00000356121

UniGene: Hs.655520