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Sheep adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱:
    綿羊促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EQ027618SH
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.097 pg/mL-400 pg/mL
  • 靈敏度:
    0.097 pg/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    綿羊促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒(CSB-EQ027618SH)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的ACTH含量。促腎上腺皮質(zhì)激素(ACTH)是腺垂體分泌的多肽激素。其它對維持腎上腺正常形態(tài)和功能有重要作用。研究機制上,ACTH作用于腎上腺皮質(zhì)束狀帶,刺激糖皮質(zhì)激素合成與分泌,在應激反應等生理過程中發(fā)揮關(guān)鍵調(diào)節(jié)作用。試劑盒檢測范圍為0.097 pg/mL-400 pg/mL,適用于綿羊等反芻動物應激狀態(tài)評估、腎上腺功能研究、藥理實驗中激素水平動態(tài)監(jiān)測等科研領(lǐng)域本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    ACTH
  • 種屬:
    Ovis aries (Sheep)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 82-89  
    1:2 Average % 91  
    Range % 88-95  
    1:4 Average % 87  
    Range % 83-95  
    1:8 Average % 100  
    Range % 95-105  
  • 回收率:
    The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-103  
    EDTA plasma (n=4) 89 87-92  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.175 0.177 0.176    
    100 0.254 0.268 0.261    
    25 0.335 0.353 0.344    
    6.25 0.582 0.598 0.590    
    1.56 0.913 0.934 0.924    
    0.39 1.461 1.498 1.480    
    0.097 1.751 1.659 1.705    
    0 2.031 1.957 1.994    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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