Rat angiotensin 1-7 (Ang1-7) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒（CSB-E14241r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Ang1-7含量。Ang1-7是一種血管緊張素，作為腎素-血管緊張素系統(tǒng)（RAS）的組成部分，其作用與AngⅡ相反。研究顯示，Ang1-7可通過調(diào)節(jié)Rac1、Rad等蛋白表達(dá)，抑制成纖維細(xì)胞合成細(xì)胞外基質(zhì)，從而對(duì)心血管系統(tǒng)產(chǎn)生保護(hù)作用。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，適用于基礎(chǔ)科研中評(píng)估心血管疾病模型、代謝紊亂或器官損傷等實(shí)驗(yàn)條件下Ang1 - 7的動(dòng)態(tài)變化，在高血壓、糖尿病腎病等疾病機(jī)制研究中具有重要價(jià)值，滿足實(shí)驗(yàn)室對(duì)生物活性分子定量分析的需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Ang1-7
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	102
	Range %	92-114
1:8	Average %	99
	Range %	80-110

回收率：

The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	83-112
EDTA plasma (n=4)	95	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.