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  4. Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

  • 中文名稱：
    大鼠磷酸肌醇3激酶(PI3K)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08418r
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠磷酸肌醇3激酶(PI3K)酶聯(lián)免疫試劑盒（CSB-E08418r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PI3K含量。PI3K（磷脂酰肌醇-3激酶）是一種脂類激酶，在癌癥發(fā)生發(fā)展中起著關鍵作用。其研究背景源于其在細胞信號傳導中的重要作用，特別是在PI3K/Akt/mTOR信號通路中，該通路調(diào)控細胞生長、存活、代謝和血管生成。PI3K通過磷酸化脂質(zhì)分子PI-3,4,5-P3（PIP3）來激活下游信號分子，如Akt和mTOR，從而促進腫瘤生長和抵抗治療。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品可廣泛應用于基礎科研領域，包括但不限于腫瘤微環(huán)境研究、代謝性疾病模型構建、藥物作用機制探索等實驗場景，為PI3K相關信號通路分析及分子調(diào)控研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    PI3K
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-95  
    1:2 Average % 103  
    Range % 99-107  
    1:4 Average % 93  
    Range % 88-94  
    1:8 Average % 99  
    Range % 95-103  
  • 回收率：
    The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.860 2.778 2.819 2.679  
    500 2.231 2.222 2.227 2.087  
    250 1.379 1.404 1.392 1.252  
    125 0.748 0.761 0.755 0.615  
    62.5 0.464 0.448 0.456 0.316  
    31.2 0.307 0.302 0.305 0.165  
    15.6 0.218 0.211 0.215 0.075  
    0 0.141 0.139 0.140 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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