Rat Motilin,MTL ELISA Kit

產品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠胃動素(MTL)酶聯免疫試劑盒（CSB-E08208r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MTL含量。大鼠胃動素（MTL）是一種由內分泌Mo細胞分泌的22個氨基酸組成的活性肽，主要在消化間期周期性釋放，促進胃腸運動和胃蛋白酶分泌。其作用機制涉及與胃腸平滑肌上的胃動素受體結合，增強平滑肌收縮，加速胃排空，并調節消化間期胃腸活動，如移行性復合運動（MMC）。MTL的研究對于理解胃腸動力和消化功能具有重要意義。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于胃腸動力障礙動物模型研究、藥物干預后胃動素分泌評估，以及消化道組織樣本中MTL表達的動態監測，為胃腸生理學、病理學機制研究及藥效學評價提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

MTL
種屬：

Rattus norvegicus (Rat)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-96
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	96-106
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-108
EDTA plasma (n=4)	96	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.