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  4. Rat Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit

Rat Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit

  • 中文名稱：
    大鼠胰島素樣生長因子結(jié)合蛋白3(IGFBP-3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07353r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.24 ng/mL-15 ng/mL
  • 靈敏度：
    0.06 ng/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰島素樣生長因子結(jié)合蛋白3(IGFBP-3)酶聯(lián)免疫試劑盒（CSB-E07353r）檢測血清、血漿、組織勻漿樣本中的IGFBP3含量。IGFBP3（胰島素樣生長因子結(jié)合蛋白3）是一種在血液中主要轉(zhuǎn)運IGF的蛋白，參與調(diào)節(jié)IGF-1和IGF-2的活性。其研究機制涉及延長IGF半衰期、抑制IGF與受體相互作用，并在細胞信號傳導(dǎo)、腫瘤抑制等方面發(fā)揮作用。試劑盒檢測范圍為0.24 ng/ml-15 ng/ml，靈敏度為0.06 ng/ml。適用于大鼠模型中的生長發(fā)育評估、代謝性疾病機制研究、營養(yǎng)狀態(tài)分析等科研場景，例如探究內(nèi)分泌紊亂模型或能量代謝干預(yù)實驗中的IGFBP - 3動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Igfbp3 ELISA Kit; Igfbp-3 ELISA Kit; Insulin-like growth factor-binding protein 3 ELISA Kit; IBP-3 ELISA Kit; IGF-binding protein 3 ELISA Kit; IGFBP-3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:20

    Average %

    84

    Range %

    80-92

    1:40

    Average %

    90

    Range %

    85-95

    1:80

    Average %

    97

    Range %

    92-107

    1:160

    Average %

    106

    Range %

    101-112

  • 回收率：

    The recovery of rat IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    86-97

    EDTA plasma (n=4)

    93

    88-101

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    15

    3.631

    3.417

    3.524

    3.441

    7.5

    3.033

    3.063

    3.048

    2.965

    3.75

    2.255

    2.223

    2.239

    2.156

    1.88

    1.221

    1.286

    1.254

    1.170

    0.94

    0.691

    0.677

    0.684

    0.601

    0.47

    0.434

    0.430

    0.432

    0.349

    0.24

    0.268

    0.274

    0.271

    0.188

    0

    0.085

    0.081

    0.083

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.
  • 基因功能參考文獻：
    1. Compound 49b treatment inhibited these retinal changes. In REC cultured in hypoxia, levels of IGFBP-3 were reduced, which were significantly increased by Compound 49b. PMID: 27439004
    2. Study indicated that IGFBP-3 shRNA might rehabilitate erectile function via decreased concentrations of serum LDL lipoprotein and triglyceride, increased cavernosal smooth muscle and improvement in NO-cGMP signaling in diabetic rats. PMID: 25879169
    3. IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats PMID: 25582342
    4. IGFBP-3 has novel protective effects on retinal and systemic vasculature and may be a therapeutic candidate for ocular complications such as diabetic retinopathy. PMID: 22792172
    5. obesity induces specific changes in the expression profile of the adipose tissue depot surrounding the pancreas, leading to a decrease in IGFBP3 secretion PMID: 22067319
    6. The results support the suggestion that inactivation of Mxi1 has a positive effect on cell proliferation by down-regulating IGFBP-3. PMID: 22008548
    7. Paraquat-treated rats showed enhanced gene expression of growth factors such as platelet-derived growth factor-A and insulin-like growth factor-1 at 21 days and transforming growth factor-beta 1 at 14 days. PMID: 20498031
    8. Data suggest that the inhibitory effect of chronic arthritis on skeletal muscle is higher in fast glycolytic than in slow oxidative muscle and that IGF-I administration attenuates this effect and decreases atrogin-1 and IGFBP-3 gene expression. PMID: 20519361
    9. Downregulation of IGFBP-3 gene expression may cause pulmonary hypoplasia in the nitrofen-induced CDH model by interfering with retinoid signaling pathway. PMID: 19844724
    10. IGFBP-3 and BAX interaction activates germ cell apoptosis via the mitochondria-dependent pathway. PMID: 19887447
    11. IGFBP-3 and -5 proteins may have potential modulatory roles in type I fiber-dominated muscles, aging, and regrowth from atrophy. PMID: 12397024
    12. role of glucocorticoids in the insulin-like growth factor binding protein (IGFBP)-3 response to endotoxin PMID: 12485840
    13. Expressed in cardiac muscle cells. Serum withdrqwal-induced increase in apoptosis of cardiac muscle cells associated with IGFBP-3. PMID: 12508914
    14. data indicate that acute inflammation inhibits serum concentrations of IGFBP-3 by decreasing its synthesis in the liver, rather than increasing its proteolysis PMID: 12581876
    15. Aminoguanidine inhibition of inducible nitric oxide synthase blocked the inhibitory effect of LPS on both IGFBP-3 serum levels and its hepatic mRNA levels. PMID: 13129855
    16. IGFBP-3 could play proapoptotic action at the myocardial level and suggest a novel role for this protein in cardiovascular dysfunction. PMID: 15055478
    17. IGF-I uses different and only partially overlapping intracellular signaling pathways for regulation of IGFBP-3 and -5 with opposing biological functions might be important for modulation of IGF bioactivity in cellular microenvironment. PMID: 15845624
    18. cross-talk between the IGFBP-3-dependent STAT-1 signaling and the TGF-beta-dependent ERK pathway that regulates mesenchymal chondroprogenitor cell proliferation PMID: 15956343
    19. In utero dibutyl phthalate leads to IGFBP3 expression in rat testis in fetal and postnatal development. PMID: 16037377
    20. retinoic acid modulates the secreted IGFBP-2 and -3 during O(2) exposure and inhibits the increase in collagen that occurs during lung injury PMID: 16055936
    21. Liver cirrhosis is accompanied by an altered spatial expression of IGFBP-3 PMID: 16098781
    22. Intracerebroventricular infusion of IGFBP-3 significantly impaired insulin action at the liver (45% increase in hepatic glucose production, P < 0.01). PMID: 17003344

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  • 亞細胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：


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