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Rat Corticosterone,CORT ELISA Kit

  • 中文名稱:
    大鼠皮質酮(CORT)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E07014r
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.2 ng/mL-40 ng/mL
  • 靈敏度:
    0.1 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠皮質酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒(CSB-E07014r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CORT含量。CORT即皮質酮,是一種重要的糖皮質激素。它參與機體應激反應與代謝調節(jié)等生理過程。研究機制上,主要通過與細胞內糖皮質激素受體結合,調控相關基因表達,影響細胞功能,在神經、內分泌等多系統(tǒng)中發(fā)揮關鍵作用。試劑盒檢測范圍為0.2 ng/mL-40 ng/mL,支持科研人員開展神經內分泌機制研究、慢性應激模型評估、代謝綜合征相關實驗及藥物干預效果驗證等領域的探索,為行為學實驗、神經藥理學研究和激素調控機制探索提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    CORT
  • 種屬:
    Rattus norvegicus (Rat)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-91  
    1:2 Average % 90  
    Range % 85-95  
    1:4 Average % 97  
    Range % 92-103  
    1:8 Average % 105  
    Range % 100-112  
  • 回收率:
    The recovery of rat CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.138 0.132 0.135    
    10 0.239 0.258 0.249    
    3.2 0.504 0.509 0.507    
    0.8 0.939 1.009 0.974    
    0.2 1.419 1.424 1.422    
    0 2.154 2.159 2.157    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    CORT是一種重要的激素,可以影響多種生理過程,如免疫和應激反應。最近的研究表明,CORT可以通過影響免疫細胞的活動來調節(jié)免疫反應。例如,在炎癥過程中,CORT可以抑制炎癥介質的產生,從而緩解炎癥反應。此外,CORT也被認為是抑制免疫系統(tǒng)過度激活的重要因素之一。最近的研究還表明,CORT可能在多種神經系統(tǒng)疾病中具有治療潛力,例如抑郁癥和焦慮癥等。


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