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Rat Bone-specific Alkaline Phosphatase (BALP) ELISA Kit

  • 中文名稱:
    大鼠骨堿性磷酸酶(BALP)酶聯免疫試劑盒
  • 貨號:
    CSB-E11865r-IS
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12U/L-200U/L
  • 靈敏度:
    1.56U/L
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 別名:
    Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
  • 縮寫:
    BALP
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 反應時間:
    3.5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism?
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 儲存條件:
    Store at 2-8°C. Please refer to protocol.
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
  • 基因功能參考文獻:
    1. he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g... PMID: 27885436
    2. Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577
    3. TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine. PMID: 24894822
    4. TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration. PMID: 25411053
    5. Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS. PMID: 24888842
    6. BMP-2 and ALP gene expression is induced by a BMP-2 gene-fibronectin-apatite composite layer PMID: 21636885
    7. The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually. PMID: 21418901
    8. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
    9. Forty SNPs in ALPL and 14 SNPs in ENPP1 genetic loci as well as pairwise haplotypes were tested for association with bone strength related traits. PMID: 19931660
    10. Characterization of rat Alpl isoforms by hplc and agar gel electrophoresis are reported. PMID: 19084045
    11. ATP-mediated DBS, forming a negative feedback loop. The duodenal epithelial brush border IAP-P2Y-HCO(3-) surface microclimate pH regulatory system effectively protects the mucosa from acid injury. PMID: 19451200

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  • 亞細胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor. Extracellular vesicle membrane; Lipid-anchor, GPI-anchor. Mitochondrion membrane; Lipid-anchor, GPI-anchor. Mitochondrion intermembrane space.
  • 蛋白家族:
    Alkaline phosphatase family
  • 數據庫鏈接:


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