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Rabbit Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

  • 中文名稱:
    兔子血小板衍生生長因子AB(PDGF-AB)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E15910Rb
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子血小板衍生生長因子AB(PDGF-AB)酶聯(lián)免疫試劑盒(CSB-E15910Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PDGF-AB含量。PDGF-AB是一種血小板衍生生長因子二聚體。其在細(xì)胞增殖、分化等生理過程及腫瘤、纖維化等疾病發(fā)生發(fā)展中起作用。研究機(jī)制上,主要通過與受體結(jié)合激活下游信號通路,影響細(xì)胞行為,目前是疾病治療靶點研究熱點之一。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,適用于體外實驗環(huán)境下對生物樣本的批量檢測,支持細(xì)胞生物學(xué)、基礎(chǔ)醫(yī)學(xué)研究中涉及組織再生、血管病變或腫瘤微環(huán)境等領(lǐng)域的 PDGF - AB 動態(tài)監(jiān)測本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    PDGF-AB
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 反應(yīng)時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %82-92
    1:2Average %87
    Range %83-95
    1:4Average %94
    Range %90-98
    1:8Average %101
    Range %96-105
  • 回收率:
    The recovery of rabbit PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8783-93
    EDTA plasma (n=4)9389-97
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.846 2.734 2.790 2.696
    502.147 2.035 2.091 1.997
    251.293 1.248 1.271 1.177
    12.50.688 0.677 0.683 0.589
    6.250.412 0.424 0.418 0.324
    3.120.278 0.289 0.284 0.190
    1.560.151 0.153 0.152 0.058
    00.093 0.094 0.094
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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