Pig cardiac troponin Ⅰ,cTn-ⅠELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒（CSB-E11187p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的cTn-I含量。cTn-I（心肌肌鈣蛋白I）是一種心肌細(xì)胞特有的調(diào)節(jié)蛋白，在心肌損傷時(shí)釋放進(jìn)入血液，成為診斷心肌損傷的重要標(biāo)志物。其研究機(jī)制涉及心肌損傷時(shí)cTn-I的釋放和檢測(cè)，對(duì)于早期診斷心肌梗死等心血管疾病具有重要意義。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域中心肌損傷相關(guān)機(jī)制研究；適用于動(dòng)物模型中心臟疾病研究、心血管藥物開發(fā)中的藥效評(píng)估，以及體外實(shí)驗(yàn)中心肌細(xì)胞損傷模型的生物標(biāo)志物檢測(cè)，為科研人員提供穩(wěn)定可靠的心肌損傷動(dòng)態(tài)監(jiān)測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

cTnⅠ
Uniprot No.：

I3LLI0
種屬：

Sus scrofa (Pig)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-102
1:2	Average %	89
	Range %	85-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	93
	Range %	83-99

回收率：

The recovery of pig cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-101
EDTA plasma (n=4)	95	86-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.