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  4. Pig Procalcitonin,PCT ELISA Kit

Pig Procalcitonin,PCT ELISA Kit

  • 中文名稱：
    豬降鈣素原(PCT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15004p
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.75 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E15004p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PCT含量。PCT（降鈣素原）是一種蛋白質(zhì)。它健康狀態(tài)下在體內(nèi)水平極低，感染時會顯著升高。其研究機(jī)制主要圍繞作為感染診斷和病情監(jiān)測的生物標(biāo)志物，在細(xì)菌感染時，機(jī)體產(chǎn)生炎癥反應(yīng)促使PCT合成增加，可輔助判斷感染嚴(yán)重程度與預(yù)后。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于科研場景中豬類疾病模型的炎癥進(jìn)程監(jiān)測、疫苗或藥物干預(yù)效果評估、免疫調(diào)控機(jī)制研究等方向，為獸醫(yī)科學(xué)、動物醫(yī)學(xué)研究及生物標(biāo)志物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    PCT
  • 種屬：
    Sus scrofa (Pig)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-92
    1:2Average %95
    Range %88-98
    1:4Average %92
    Range %89-96
    1:8Average %93
    Range %88-98
  • 回收率：
    The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-96
    EDTA plasma (n=4)9692-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    25002.165 2.145 2.155 2.056
    12501.697 1.678 1.688 1.589
    6251.298 1.223 1.261 1.162
    312.50.860 0.854 0.857 0.758
    1560.516 0.501 0.509 0.410
    780.381 0.371 0.376 0.277
    390.235 0.222 0.229 0.130
    00.100 0.098 0.099 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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