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  4. Mouse plasma kallikrein(KLKB1) ELISA Kit

Mouse plasma kallikrein(KLKB1) ELISA Kit

  • 中文名稱：
    小鼠血漿激肽釋放酶(KLKB1) 酶聯免疫試劑盒
  • 貨號：
    CSB-E16637m-IS
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.3125 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 別名：
    Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2000

    Average %

    92

    Range %

    88-98

    1:4000

    Average %

    88

    Range %

    82-92

    1:8000

    Average %

    97

    Range %

    92-106

    1:16000

    Average %

    98

    Range %

    92-105

  • 回收率：

    The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-99

    EDTA plasma (n=4)

    87

    81-95

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    40

    2.745

    2.662

    2.704

    2.698

    20

    1.974

    2.012

    1.993

    1.988

    10

    1.334

    1.285

    1.309

    1.304

    5

    0.869

    0.866

    0.867

    0.862

    2.5

    0.594

    0.584

    0.589

    0.584

    1.25

    0.343

    0.363

    0.353

    0.348

    0.625

    0.191

    0.187

    0.189

    0.184

    0

    0.006

    0.006

    0.006

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • 基因功能參考文獻：
    1. Plasma kallikrein mediates brain hemorrhage and edema caused by tissue plasminogen activator therapy in mice after stroke. PMID: 28130211
    2. we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
    3. Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. PMID: 22398951
    4. PKK or fXII deficiency reduced thrombus formation in both arterial and venous thrombosis models, without an apparent effect on hemostasis. PMID: 21821705
    5. the prekallikrein gene (Klkb1) is expressed highly in the mammary gland during stromal remodeling periods including puberty and postlactational involution. PMID: 19297327

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 數據庫鏈接：


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