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Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

  • 中文名稱(chēng)：
    小鼠基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07417m
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒（CSB-E07417m）檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP-1含量。MMP-1，也稱(chēng)為間質(zhì)膠原酶和成纖維細(xì)胞膠原酶，是基質(zhì)金屬蛋白酶家族的一員。它參與細(xì)胞外基質(zhì)的降解，在正常生理過(guò)程和疾病過(guò)程中發(fā)揮作用。MMP-1通過(guò)降解膠原蛋白和明膠等成分，影響細(xì)胞微環(huán)境，與腫瘤侵襲、轉(zhuǎn)移和骨關(guān)節(jié)炎等疾病相關(guān)。研究MMP-1有助于開(kāi)發(fā)針對(duì)相關(guān)疾病的治療策略。試劑盒檢測(cè)范圍為47 pg/mL-3000 pg/mL，適用于體外實(shí)驗(yàn)研究，可支持細(xì)胞功能探索、疾病模型建立及藥物作用機(jī)制分析等科研場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
  • 縮寫(xiě)：
    MMP-1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-95  
    1:40 Average % 100  
    Range % 97-103  
    1:80 Average % 91  
    Range % 87-95  
    1:160 Average % 97  
    Range % 93-100  
  • 回收率：
    The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 90-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    3000 2.482 2.510 2.496 2.317  
    1500 1.855 1.786 1.821 1.642  
    750 1.246 1.232 1.239 1.060  
    375 0.748 0.774 0.761 0.582  
    187.5 0.495 0.456 0.476 0.297  
    93.75 0.322 0.339 0.331 0.152  
    46.88 0.253 0.242 0.248 0.069  
    0 0.180 0.178 0.179 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：血清

樣品信息：小鼠

稀釋比：其他 1:10

產(chǎn)品評(píng)價(jià)： 我用CSB-E07417m檢測(cè)小鼠血清MMP-1水平，其OD如圖所示，試劑盒樣本充足，使用說(shuō)明簡(jiǎn)潔明了，操作容易上手，有助于實(shí)驗(yàn)進(jìn)展，滿(mǎn)意。

By 劉老師

靶點(diǎn)詳情

  • 功能：
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Able to degrade synthetic peptides and type I and II fibrillar collagen.
  • 基因功能參考文獻(xiàn)：
    1. The difficulty to effectively maintain Mmp1a in the zymogen form may account for the tight control of Mmp1a expression and reduced expression in normal tissue as compared to inflammatory states or cancer. PMID: 24737602
    2. Stromal Mmp1a drives in vivo tumorigenesis. PMID: 23708660
    3. Matrix metalloproteinase Mmp-1a is not required for growth and fertility in mice and has a role in lung cancer progression by modulating inflammatory responses PMID: 23548910
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫(kù)鏈接：


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