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  4. Mouse Eotaxin 3 ELISA Kit

Mouse Eotaxin 3 ELISA Kit

  • 中文名稱：
    小鼠嗜酸粒細胞趨化蛋白Eotaxin 3(Eotaxin 3)酶聯免疫試劑盒
  • 貨號：
    CSB-E15062m
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠嗜酸粒細胞趨化蛋白Eotaxin 3(Eotaxin 3)酶聯免疫試劑盒（CSB-E15062m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的Eotaxin 3含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于研究炎癥性疾病模型中嗜酸性粒細胞募集機制、評估實驗性抗過敏藥物對趨化因子表達的調控作用，或解析免疫細胞與微環境相互作用的分子基礎，為呼吸道疾病、皮膚過敏等病理機制研究提供可靠工具。試劑盒操作流程標準化，適用于體外實驗環境下多種生物樣本的平行檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    Eotaxin 3
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Eotaxin 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %90-93
    1:2Average %96
    Range %92-100
    1:4Average %103
    Range %101-105
    1:8Average %89
    Range %84-94
  • 回收率：
    The recovery of mouse Eotaxin 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9290-94
    EDTA plasma (n=4)8985-94
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.927 2.874 2.901 2.745
    2002.015 2.135 2.075 1.919
    1001.303 1.403 1.353 1.197
    500.801 0.822 0.812 0.656
    250.532 0.524 0.528 0.372
    12.50.319 0.332 0.326 0.170
    6.250.244 0.254 0.249 0.093
    00.151 0.160 0.156
  • 數據處理：
  • 貨期：
    3-5 working days

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