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Monkey Interleukin-8,IL-8 ELISA Kit

  • 中文名稱:
    猴子白介素8(IL-8/CXCL8)酶聯免疫試劑盒
  • 貨號:
    CSB-E08051Mo-IS
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    7.8pg/mL-500pg/mL
  • 靈敏度:
    3.9pg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 別名:
    CXCL8 ELISA kit; IL8Interleukin-8 ELISA kit; IL-8 ELISA kit; C-X-C motif chemokine 8 ELISA kit; Chemokine ELISA kit; C-X-C motif) ligand 8 ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Monkey
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-8/CXCL8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:5

    Average %

    88

    Range %

    84-95

    1:10

    Average %

    92

    Range %

    85-99

    1:20

    Average %

    97

    Range %

    90-104

    1:40

    Average %

    103

    Range %

    99-109

  • 回收率:

    The recovery of monkey IL-8/CXCL8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-99

    EDTA plasma (n=4)

    97

    90-104

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.654

    2.588

    2.621

    2.449

    250

    1.578

    1.637

    1.608

    1.436

    125

    1.187

    1.098

    1.143

    0.971

    62.5

    0.683

    0.719

    0.701

    0.529

    31.2

    0.487

    0.471

    0.479

    0.307

    15.6

    0.335

    0.343

    0.339

    0.167

    7.8

    0.295

    0.278

    0.287

    0.115

    0

    0.174

    0.170

    0.172

     

  • 數據處理:
  • 貨期:
    3-5 working days.

產品評價

靶點詳情

  • 功能:
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能參考文獻:
    1. Stress decreased IL-8 gene expression in both CeA lesioned and non-lesioned animals. But, CeA lesions increased the tissue expression of IL-8 mRNA prior to and after stress exposure. PMID: 16574374
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Intercrine alpha (chemokine CxC) family
  • 數據庫鏈接:


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