Human Thromboxane B2,TX-B2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血栓素B2(TXB2)酶聯(lián)免疫試劑盒（CSB-E08046h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TX-B2含量。TX-B2，即血栓素B2，是一種由血小板合成的脂質(zhì)過氧化物，參與調(diào)節(jié)血管收縮和血小板聚集。其背景研究涉及血栓形成和心血管疾病。研究機(jī)制主要關(guān)注TX-B2在血栓形成過程中的作用，以及如何通過調(diào)節(jié)TX-B2水平來預(yù)防和治療相關(guān)疾病。試劑盒檢測(cè)范圍為9.38 pg/mL-600 pg/mL，該產(chǎn)品廣泛應(yīng)用于心血管疾病機(jī)制研究、抗血小板藥物藥效評(píng)估、炎癥信號(hào)通路探索等科研領(lǐng)域，例如通過檢測(cè)阿司匹林干預(yù)后血小板釋放的 TXB2 水平變化，或分析動(dòng)脈粥樣硬化模型動(dòng)物組織中的血栓素代謝動(dòng)態(tài)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TX-B2
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	80-105
1:2	Average %	95
Range %	85-108
1:4	Average %	106
Range %	90-112
1:8	Average %	95
Range %	80-110

回收率：

The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	80-110
EDTA plasma (n=4)	98	90-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.