Human Pentosidine ELISA Kit

產(chǎn)品詳情

文獻引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09415h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、尿液樣本中的Pentosidine含量。Pentosidine是一個靶點，它屬于晚期糖基化終末產(chǎn)物（AGEs）。在體內(nèi)，它主要通過美拉德反應形成并逐漸積累。研究機制方面，它與糖尿病并發(fā)癥、衰老等密切相關(guān)，可引發(fā)氧化應激和炎癥反應，常作為評估相關(guān)疾病風險和進程的生物標志物。試劑盒檢測范圍為31.25 pmol/mL-2000 pmol/mL，該產(chǎn)品適用于基礎醫(yī)學研究、疾病機制探索、藥物干預效果評估等科研場景，為代謝性疾病、衰老相關(guān)疾病及骨關(guān)節(jié)健康研究提供可靠的生物標志物檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Pentosidine
種屬：

Homo sapiens (Human)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	81-102
1:2	Average %	95
	Range %	84-105
1:4	Average %	104
	Range %	91-111
1:8	Average %	95
	Range %	80-103

回收率：

The recovery of human Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-105
EDTA plasma (n=4)	94	88-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.