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  4. Human Lipoxin A4,LXA4 ELISA Kit

Human Lipoxin A4,LXA4 ELISA Kit

  • 中文名稱：
    人脂氧素A4(LXA4)酶聯免疫試劑盒
  • 貨號：
    CSB-E09689h
  • 樣本類型：
    serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
  • 檢測范圍：
    0.625 pg/mL-40 pg/mL
  • 靈敏度：
    0.156 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人脂氧素A4(LXA4)酶聯免疫試劑盒（CSB-E09689h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、尿液、組織勻漿、細胞裂解物樣本中的LXA4含量。LXA4（脂氧素A4）是一種內源性抗炎和免疫調節介質，由花生四烯酸通過脂氧合酶作用合成。它通過抑制炎癥細胞、調節促炎和抗炎因子平衡，限制炎癥損傷，在多種生理和病理過程中發揮重要作用。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，該產品可用于體外研究炎癥相關信號通路、評估細胞模型或動物模型中LXA4的動態變化，或探索其在組織微環境調控中的作用，為炎癥性疾病機制研究提供工具支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    LXA4
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %88
    Range %84-92
    1:200Average %94
    Range %90-98
    1:400Average %99
    Range %95-104
    1:800Average %95
    Range %88-99
  • 回收率：
    The recovery of human LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9487-99
    EDTA plasma (n=4)10095-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.433 2.374 2.404 2.265
    201.947 1.879 1.913 1.774
    101.256 1.307 1.282 1.143
    50.787 0.811 0.799 0.660
    2.50.546 0.522 0.534 0.395
    1.250.392 0.401 0.397 0.258
    0.6250.246 0.255 0.251 0.112
    00.135 0.142 0.139 　
  • 數據處理：
  • 貨期：
    3-5 working days

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