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Human Homocysteic acid,Hcy ELISA Kit

  • 中文名稱(chēng):
    人同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08895h
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates, urine
  • 檢測(cè)范圍:
    0.78 nmol/mL-50 nmol/mL
  • 靈敏度:
    0.195 nmol/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷(xiāo):
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒(CSB-E08895h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、尿液樣本中的Hcy含量。Hcy即同型半胱氨酸,是蛋氨酸代謝中間產(chǎn)物。其水平異常升高被視為心腦血管疾病等的獨(dú)立危險(xiǎn)因素。它會(huì)損傷血管內(nèi)皮細(xì)胞,促進(jìn)血栓形成,影響脂質(zhì)代謝,還能引發(fā)氧化應(yīng)激反應(yīng),干擾正常細(xì)胞功能。試劑盒檢測(cè)范圍為0.78 nmol/mL-50 nmol/mL,本產(chǎn)品適用于疾病分子機(jī)制研究、營(yíng)養(yǎng)代謝分析、藥物干預(yù)效果評(píng)估及生物標(biāo)志物篩查等科研場(chǎng)景,為實(shí)驗(yàn)室提供標(biāo)準(zhǔn)化、高重復(fù)性的Hcy檢測(cè)方案,僅限科學(xué)研究使用。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    N/A
  • 縮寫(xiě):
    Hcy
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %98
    Range %91-105
    1:4Average %89
    Range %84-96
    1:8Average %93
    Range %86-98
  • 回收率:
    The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-101
    EDTA plasma (n=4)9289-94
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    502.082 2.156 2.119 1.997
    251.456 1.489 1.473 1.351
    12.50.941 0.968 0.955 0.833
    6.250.583 0.597 0.590 0.468
    3.1250.342 0.367 0.355 0.233
    1.560.248 0.261 0.255 0.133
    0.780.192 0.198 0.195 0.073
    00.121 0.123 0.122  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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