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  4. Human Dentin Matrix Protein 1 (DMP1) ELISA kit

Human Dentin Matrix Protein 1 (DMP1) ELISA kit

  • 中文名稱：
    人牙本質基質蛋白1(DMP1)酶聯免疫試劑盒
  • 貨號：
    CSB-E13029h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人牙本質基質蛋白1(DMP1)酶聯免疫試劑盒（CSB-E13029h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DMP1含量。人牙本質基質蛋白1（DMP1）是一種在牙齒發育和生物礦化過程中起關鍵作用的蛋白質。它在牙本質形成、礦化及牙本質修復等過程中發揮著重要作用，同時也是常染色體隱性低血磷性佝僂病和非礦化組織疾病發病機制研究的重要靶點。DMP1通過調節羥磷灰石的成核作用啟動礦化過程，并參與細胞間信號傳遞。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研領域中對牙齒發育機制、骨組織再生調控或生物材料礦化效應的研究，為探索礦化相關疾病的分子機制或組織工程研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    DMP1 ELISA Kit; Dentin matrix acidic phosphoprotein 1 ELISA Kit; DMP-1 ELISA Kit; Dentin matrix protein 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 81-91  
    1:2 Average % 102  
    Range % 98-105  
    1:4 Average % 105  
    Range % 98-108  
    1:8 Average % 96  
    Range % 92-98  
  • 回收率：
    The recovery of human DMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-95  
    EDTA plasma (n=4) 96 92-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.688 2.722 2.705 2.586  
    10 2.399 2.292 2.346 2.227  
    5 1.894 1.886 1.890 1.771  
    2.5 1.074 1.061 1.068 0.949  
    1.25 0.778 0.788 0.783 0.664  
    0.625 0.399 0.386 0.393 0.274  
    0.312 0.263 0.248 0.256 0.137  
    0 0.118 0.120 0.119 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
  • 基因功能參考文獻：
    1. dentin matrix protein 1 was detected in the periodontal ligament co-localized with periostin in the vicinity of the cement PMID: 29289709
    2. The Dentin matrix protein1 (Dmp1) is an acidic phosphoprotein of osteocytes proposed to participate in bone and dentin mineralization. PMID: 28247029
    3. Thus, unspDMP-1 is a novel chromatin-enriched RNA that epigenetically regulates cellular proliferation of SQCC. PMID: 29278708
    4. DMP1 might be a potential factor contributing to cardiovascular complications in dialysis patients. PMID: 27390906
    5. DMP1 gene seems to be involved in genetic predisposition to Ankylosing spondylitis (AS). PMID: 26960368
    6. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials. PMID: 25678029
    7. This review will focus on the aberrant splicing of tumor suppressor/oncogenes that belong to the DMP1-ARF-MDM2-p53 pathway PMID: 26802432
    8. Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences PMID: 25578972
    9. identify alternative splicing as a mechanism utilized by cancer cells to modulate the DMP1 locus through diminishing DMP1alpha tumour suppressor expression PMID: 25537728
    10. The findings indicate that DMP-1 is a useful marker of osteogenic differentiation and mineralisation in soft tissue tumours PMID: 25630512
    11. A family of autosomal recessive form of Hypophosphatemic rickets secondary to a DMP1 mutation located in the first coding exon of the gene, is reported. PMID: 25180662
    12. Results suggest that FAM20C suppresses FGF23 production by enhancing DMP1 expression, and inactivating mutations in FAM20C cause FGF23-related hypophosphatemia by decreasing transcription of DMP1. PMID: 25026495
    13. DMP1 may play an important role in maintaining the chondrogenic phenotype and its possible involvement in altered cartilage matrix remodelling and degradation in disease conditions like osteoarthritis. PMID: 24987156
    14. DMP1 and DSPP were more abundant in carious than in sound samples. PMID: 24441185
    15. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours. PMID: 23559304
    16. Mutations in PHEX and DMP1 play a role in causing hypophosphatemic rickets. PMID: 22695891
    17. DMP1 is regulated post-transcriptionally by let-7 during odontoblast differentiation. PMID: 22552299
    18. DMP-1 stimulated production of IL-6/IL-8 from pulp fibroblasts. Inhibition of p38 mitogen-activated protein kinase pathway blocked proinflammatory effect on pulp fibroblasts. DMP-1 might participate in development of inflammatory changes in pulp. PMID: 22152625
    19. Simvastatin plus all-trans retinoic acid reduces expression of WT1 and DMP1 in the promyelocytic leukemia cell line NB4. PMID: 22093791
    20. DMP1 is specifically involved in signaling via extracellular matrix-cell surface interaction. PMID: 21642437
    21. Data show the wide spectrum of genetic variation that can be seen in PHEX, FGF23 and DMP1 when screening a large cohort with hypophosphatemic rickets. PMID: 21050253
    22. after VE-cadherin induction by DMP1, DMP1 inhibited VEGFR-2 phosphorylation and Src-mediated signaling; DMP1 is identified as a new specific inhibitor of VEGF-induced angiogenesis PMID: 21190990
    23. possible role of dentine matrix protein 1 in the aetiology of [aggressive periodontitis] PMID: 21299946
    24. clinical and laboratory observations in this family confirm that DMP1 has an important role in normal skeletal development and mineral homeostasis PMID: 20499351
    25. Study shows the pivotal roles of Dmp1 in HER2/neu-p53 signaling and breast carcinogenesis. PMID: 21062982
    26. study describes a Japanese family that includes 2 autosomal recessive hypophosphatemic rickets-affected siblings carrying a novel mutation of the DMP1 gene (98G>A, W33X), which leads to a truncated DMP protein with no putative biological function PMID: 20213538
    27. The identified genetic mutation underscores the importance of DMP-1 mutations in the pathogenesis of autosomal recessive form of hypophosphatemia. PMID: 19796717
    28. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
    29. Three SIBLINGs (small integrin-binding ligand, N-linked glycoproteins) enhance factor H's cofactor activity enabling MCP-like cellular evasion of complement-mediated attack. PMID: 11825898
    30. DMP1 immunostaining intensity and extent scores were significantly higher in adenocarcinoma (p = 0.0004) and squamous carcinoma (p < 0.0001) samples compared with adjacent normal lung tissue PMID: 12929940
    31. dentin matrix protein-1 is processed by Bone morphogenetic protein-1/Tolloid-like proteinases PMID: 14578349
    32. Findings indicate that DMP1 immunohistochemistry is a useful tool for identifying phosphaturic mesenchymal tumors (PMTs). PMID: 15001995
    33. a silencing cis-element may play a critical role in the regulation of DMP1 cell-specific expression PMID: 15108359
    34. 8-kb region of the Dentin matrix protein 1 (DMP1) gene is a target for mechanotransduction in osteocytes, and its cis-regulatory activity may be correlated to local strain in bone PMID: 15728181
    35. Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44 PMID: 16357164
    36. analysis of a chondroitin sulfate chain attached to the bone dentin matrix protein 1 NH2-terminal fragment PMID: 16421105
    37. DMP1 regulates the expression of the DSPP gene PMID: 16679514
    38. Absence of DMP1 results in defective osteocyte maturation and increased FGF23 expression, leading to pathological changes in bone mineralization. PMID: 17033621
    39. DMP1 may regulate FGF23 expression. PMID: 17033625
    40. DMP1 has a role in breast cancer progression and bone metastases development PMID: 17136477
    41. Thus, our results suggest that Cbfalpha1 upregulates DMP1 gene expression differentially that may contribute to the spatial-temporal expression pattern of DMP1 during odontoblast differentiation. PMID: 17434448
    42. Discussion of DMP1 role in the maturation of odontoblasts and osteoblasts as well as in mineralization; identification of DMP1 mutations in autosomal-recessive hypophosphatemic rickets; and regulation of phosphate homeostasis through FGF23 [review]. PMID: 18037646
    43. mRNA of DMP1 involved in the pathogenesis of hypophosphataemic rickets is highly expressed in cells of the osteoblasts/osteocyte lineage. PMID: 18214537
    44. inactivating mutations in DMP1 increase, through yet unknown mechanisms, FGF23 synthesis and thus enhance renal phosphate excretion--REVIEW PMID: 18660670
    45. binding of DMP1 with GRP-78 receptor might be an important mechanism by which DMP1 is internalized and transported to the nucleus during bone and tooth development PMID: 18757373
    46. Dentin matrix protein-1 isoforms promote differential cell attachment and migration PMID: 18819913
    47. A novel DMP1 deletion is identified as the cause of autosomal recessive hypophosphatemic rickets, as well as demonstrated that the ARHR mutations alter DMP1 cellular processing, and that DMP1 can be regulated by vitamin D. PMID: 19007919
    48. Deletion of mouse Dmp1 results in bone hypomineralization. PMID: 16294270
    49. DMP1 is expressed in salivary gland ducts. PMID: 15329369
    50. DMP1 is expressed in parietal cells of Bowman's capsule and throughout the ductal system of nephrons. PMID: 15954904

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  • 相關疾?。?/div>
    Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1)
  • 亞細胞定位：
    Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
  • 組織特異性：
    Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
  • 數據庫鏈接：

    HGNC: 2932

    OMIM: 241520

    KEGG: hsa:1758

    STRING: 9606.ENSP00000340935

    UniGene: Hs.652366



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