Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

產品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒（CSB-E05140h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。它是診斷急性心肌梗死等心臟疾病的重要生物標志物。研究機制是當心肌受損時，CK-MB會釋放進入血液，通過檢測血中其含量變化，可輔助判斷心肌損傷程度、范圍及病情發展。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，本試劑盒適用于科研領域中心肌損傷相關疾病模型的機制探索，如心肌梗死、心肌炎或藥物心臟毒性評估等研究場景，可通過檢測不同樣本類型（如血液樣本動態監測病程進展，尿液樣本探索新型標志物潛力，或組織勻漿分析離體心肌損傷）為心血管病理研究提供定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

CK-MB
種屬：

Homo sapiens (Human)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	94
	Range %	89-100
1:4	Average %	98
	Range %	91-104
1:8	Average %	106
	Range %	102-112

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
60	3.334	3.234	3.284	3.168
30	2.152	2.067	2.110	1.994
15	1.214	1.168	1.191	1.075
7.5	0.743	0.729	0.736	0.620
3.75	0.443	0.451	0.447	0.331
1.8	0.289	0.273	0.281	0.165
0.9	0.198	0.187	0.192	0.077
0	0.113	0.118	0.116