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Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

  • 中文名稱(chēng):
    人Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E17116h
  • 樣本類(lèi)型:
    serum, plasma, cell culture supernates, urine
  • 檢測(cè)范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷(xiāo):
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒(CSB-E17116h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液樣本中的Col Ⅳ含量。Col Ⅳ(IV型膠原蛋白)是細(xì)胞基底膜的主要結(jié)構(gòu)成分,參與組織完整性維持和正常功能。其研究機(jī)制涉及細(xì)胞外基質(zhì)組成、細(xì)胞黏附、遷移、生存、擴(kuò)張和分化。在疾病如腫瘤、肝纖維化等中,Col Ⅳ的表達(dá)和功能異常,成為治療研究的重要靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL,本產(chǎn)品適用于體外研究基底膜相關(guān)疾病機(jī)制、評(píng)估藥物對(duì)細(xì)胞外基質(zhì)代謝的影響,或通過(guò)體液樣本分析Col Ⅳ作為生物標(biāo)志物的潛在價(jià)值,為組織修復(fù)、血管生成及纖維化研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
  • 縮寫(xiě):
    Col Ⅳ
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 86  
    Range % 80-104  
    1:400 Average % 97  
    Range % 91-105  
    1:800 Average % 100  
    Range % 92-113  
    1:1600 Average % 93  
    Range % 86-98  
  • 回收率:
    The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-102  
    EDTA plasma (n=4) 93 85-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.748 2.856 2.802 2.635  
    2000 2.459 2.436 2.448 2.281  
    1000 1.876 1.968 1.922 1.755  
    500 1.240 1.234 1.237 1.070  
    250 0.843 0.836 0.840 0.673  
    125 0.434 0.447 0.441 0.274  
    62.5 0.246 0.256 0.251 0.084  
    0 0.165 0.168 0.167    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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