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  4. Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名稱：
    人Ⅰ型膠原(Col Ⅰ)酶聯免疫試劑盒
  • 貨號：
    CSB-E08082h-IS
  • 樣本類型：
    serum, plasma, cell culture supernates, saliva, tissue homogenates
  • 檢測范圍：
    0.24ng/mL-15ng/ml
  • 靈敏度：
    0.12ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 別名：
    Collagen Type 1,Col 1,Collagen Type I,Col I
  • 縮寫：
    Col Ⅰ
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:20

    Average %

    92

    Range %

    88-97

    1:40

    Average %

    100

    Range %

    95-106

    1:80

    Average %

    94

    Range %

    88-99

    1:160

    Average %

    103

    Range %

    99-109

  • 回收率：

    The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    85-96

    EDTA plasma (n=4)

    97

    92-103

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    15

    3.400

    3.234

    3.317

    3.155

    7.5

    2.809

    2.627

    2.718

    2.556

    3.75

    1.692

    1.788

    1.740

    1.578

    1.88

    1.024

    1.029

    1.027

    0.864

    0.94

    0.609

    0.621

    0.615

    0.453

    0.47

    0.411

    0.403

    0.407

    0.245

    0.24

    0.293

    0.287

    0.290

    0.128

    0

    0.168

    0.157

    0.163

    　

  • 數據處理：
  • 貨期：
    3-5 working days.

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