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  4. Human Carboxypeptidase E(CPE) ELISA kit

Human Carboxypeptidase E(CPE) ELISA kit

  • 中文名稱：
    人羧肽酶E(CPE)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005886HU
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    39.06 pg/mL-2500 pg/mL
  • 靈敏度：
    9.76 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人羧肽酶E(CPE)酶聯(lián)免疫試劑盒（CSB-EL005886HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CPE含量。CPE即羧肽酶E，是一種與神經(jīng)內(nèi)分泌相關(guān)的酶。其背景在于在腦和內(nèi)分泌組織中廣泛存在。研究機(jī)制上，它參與神經(jīng)肽和激素前體加工，對(duì)調(diào)節(jié)神經(jīng)遞質(zhì)釋放、內(nèi)分泌信號(hào)傳導(dǎo)等有重要作用，在神經(jīng)生理和內(nèi)分泌相關(guān)疾病研究方面受關(guān)注。試劑盒檢測(cè)范圍為39.06 pg/mL-2500 pg/mL，適用于科研場(chǎng)景下探究CPE在代謝性疾病模型、神經(jīng)內(nèi)分泌調(diào)控機(jī)制中的表達(dá)變化，亦可用于評(píng)估藥物干預(yù)或基因修飾對(duì)CPE活性的影響。為研究CPE在病理生理過(guò)程中的分子機(jī)制及其作為潛在生物標(biāo)志物的探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Carboxypeptidase E ELISA Kit; Carboxypeptidase H ELISA Kit; Carboxypeptidase H precursor ELISA Kit; CarboxypeptidaseE ELISA Kit; CarboxypeptidaseH ELISA Kit; CBPE_HUMAN ELISA Kit; Cobalt stimulated chromaffin granule carboxypeptidase ELISA Kit; CPE ELISA Kit; Cph 1 ELISA Kit; CPH ELISA Kit; Enkephalin convertase ELISA Kit; Entephalin convertase ELISA Kit; Insulin granule associated carboxypeptidase ELISA Kit; Prohormone processing carboxypeptidase ELISA Kit; Prohormone-processing carboxypeptidase ELISA Kit
  • 縮寫：
    CPE
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 94-109
    1:2 Average % 107
    Range % 98-111
    1:4 Average % 92
    Range % 84-95
    1:8 Average % 98
    Range % 90-102
  • 回收率：
    The recovery of human CPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 84-94
    EDTA plasma (n=4) 101 95-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2500 2.410 2.333 2.372 2.246
    1250 1.712 1.679 1.696 1.570
    625 1.029 0.968 0.999 0.873
    312.5 0.634 0.655 0.645 0.519
    156.25 0.410 0.401 0.406 0.280
    78.12 0.280 0.284 0.282 0.156
    39.06 0.218 0.225 0.222 0.096
    0 0.125 0.127 0.126 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
  • 基因功能參考文獻(xiàn)：
    1. Our results implicate a novel role for Carboxypeptidase E that mainly affects the expression of motility-associated genes via several signal pathways PMID: 28656234
    2. This study has uncovered a human CPE/NF-alpha1 gene mutation that could lead to comorbidity of dementia and depression, emphasizing the importance of this gene in cognitive function. PMID: 27922637
    3. CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function. PMID: 27375026
    4. we have identified a novel SNP in the CPE gene which results in the loss of its neuroprotective function in cells and may confer neurological disorders in humans PMID: 28114332
    5. High-level CPE [ carboxypeptidase E] expression was associated with a poor prognosis in early-stage cervical cancer. CPE may serve as a biomarker for predicting PLNM [ pelvic lymph node metastasis ] and survival in these patients. PMID: 26695643
    6. Low carboxypeptidase E expression is associated with recurrence in early-stage hepatocellular carcinoma. PMID: 26803519
    7. Down-expression of liver carboxypeptidase E may reduce the secretion of serum cholecystokinin and contribute to the formation of cholesterol gallstone. PMID: 26228366
    8. Downregulation of CPE regulates cell proliferation and chemosensitivity in pancreatic cancer. PMID: 25374060
    9. Disruption of insulin receptor (IR) expression in beta cells has a direct impact on the expression of the convertase enzyme carboxypeptidase E (CPE) by inhibition of the eukaryotic translation initiation factor Eif4g1. PMID: 24843127
    10. Upregulation of CPE promotes cell proliferation and tumorigenicity in colorectal cancer. PMID: 24006921
    11. Data suggest that splice variant of carboxypeptidase E (CPE-DeltaN) that CPE-DeltaN expression might be a potential prognostic marker for colorectal cancer patients. PMID: 23852859
    12. CPE is essential in the process and targeting of neuropeptides and neurotrophins, its participation in the pathological progression of Alzheimer's disease may be suggested PMID: 22998035
    13. CPE forms a complex, probably through sequences located at its N-terminal domain, with Wnt3a and the extracellular cysteine rich domain of Fz1. PMID: 22824791
    14. Neither high glucose nor insulin (with low glucose) regulates beta-cell CPE (but either up-regulates CPD). PMID: 21628999
    15. an N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers PMID: 21285511
    16. CPE may play a role in promoting tumor growth and invasion. PMID: 21061162
    17. Carboxypeptidase E is differently expressed in subcutaneous and visceral fat of obese subjects PMID: 12530526
    18. protein binding with Con A in seminal plasma PMID: 14690244
    19. cDNA microarray analysis led to the identification of 2 novel biomarkers that should facilitate molecular diagnosis and further study of pulmonary neuroendocrine tumors. PMID: 15492986
    20. A possible role for mutations in CPE in the development of coronary heart disease. PMID: 17957445
    21. the severity of the coronary atherosclerosis estimated by Gensini score was significantly influenced by the presence of the A2925G mutant and G2855A mutant of the CPE gene PMID: 18080843
    22. polymorphism in the CPE exon5 gene may contribute to the angiographical characteristics of coronary atherosclerosis in the Chinese population PMID: 18501121
    23. Carboxypeptidase E degradation contributes to palmitate-induced beta-cell ER stress and apoptosis; CPE is a major link between hyperlipidemia and beta-cell death pathways in diabetes. PMID: 18550819
    24. CPH-Abs may allow discrimination of a more latent subset of adult-onset autoimmune diabetes (LADA). PMID: 19120309
    25. Carboxypeptidase E may be a key molecule to regulate Caspr2 (carboxypeptidase E) trafficking to the cell membrane. PMID: 19166515
    26. missense polymorphism encoding altered activity PMID: 11462236

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  • 亞細(xì)胞定位：
    [Isoform 1]: Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted.
  • 蛋白家族：
    Peptidase M14 family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2303

    OMIM: 114855

    KEGG: hsa:1363

    STRING: 9606.ENSP00000386104

    UniGene: Hs.75360



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