Human 17-Hydroxyprogesterone,17-OHP ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人17羥孕酮(17-OHP)酶聯免疫試劑盒（CSB-E05087h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的17-OHP含量。17-OHP即17-羥孕酮，與先天性腎上腺皮質增生癥等疾病密切相關。其背景在于它是腎上腺皮質激素合成過程的中間產物。研究機制主要聚焦于通過檢測其在血液中的水平來輔助診斷相關疾病，為臨床治療提供重要依據。試劑盒檢測范圍為0.625 ng/ml-10 ng/ml，靈敏度為0.625ng/ml。適用于內分泌疾病機制研究、激素代謝通路探索、藥物干預效果評估等科研領域，特別在腎上腺相關動物模型構建、類固醇激素代謝組學分析及生殖內分泌研究中可提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

17-OHP
種屬：

Homo sapiens (Human)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 17-OHP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	91
	Range %	86-96
1:4	Average %	94
	Range %	89-100
1:8	Average %	89
	Range %	85-95

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average
10	0.299	0.314	0.306
5	0.427	0.407	0.417
2.5	0.628	0.612	0.620
1.25	0.988	0.942	0.965
0.625	1.604	1.611	1.607
0	2.720	2.873	2.797