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Horse Prolactin(PRL) ELISA kit

  • 中文名稱:
    馬催乳素(PRL)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL018724HO
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    2.5 ng/mL-1000 ng/mL
  • 靈敏度:
    2.5 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    馬催乳素(PRL)酶聯(lián)免疫試劑盒(CSB-EL018724HO)為競爭法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的PRL含量。PRL即泌乳素,是腺垂體分泌的多肽激素。其分泌受下丘腦調(diào)節(jié)。研究機(jī)制上,它在乳腺發(fā)育、泌乳啟動(dòng)維持等生理過程中發(fā)揮重要作用,異常分泌會(huì)致月經(jīng)不調(diào)、不孕等,在腫瘤發(fā)生發(fā)展等方面也有一定關(guān)聯(lián),是醫(yī)學(xué)研究重要靶點(diǎn)。試劑盒檢測(cè)范圍為2.5 ng/mL-1000 ng/mL,產(chǎn)品經(jīng)過嚴(yán)格的性能驗(yàn)證,可穩(wěn)定應(yīng)用于馬科動(dòng)物繁殖生理、泌乳調(diào)控機(jī)制、應(yīng)激狀態(tài)評(píng)估等研究領(lǐng)域,為畜牧科研、動(dòng)物內(nèi)分泌研究及生殖健康管理提供可靠的數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Equus caballus (Horse)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-101
    1:2 Average % 95
    Range % 85-100
    1:4 Average % 91
    Range % 89-99
    1:8 Average % 93
    Range % 90-100
  • 回收率:
    The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-105
    EDTA plasma (n=4) 97 88-102
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    0 2.133 2.121 2.127
    2.5 1.516 1.501 1.509
    8.75 0.996 1.002 0.999
    31.25 0.538 0.555 0.547
    187.5 0.341 0.339 0.340
    1000 0.162 0.159 0.161
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

 常見問題解答
Q:

我想要確定這個(gè)CSB-EL018724HO Horse Prolactin(PRL) ELISA kit是否適用于犀牛樣品?用什么抗原來制造捕獲抗體?捕獲抗體是單克隆的還是多克隆的?競爭性抗原是什么?

A:
CSB-EL018724HO酶標(biāo)板上包被山羊抗兔抗體(免疫原為兔IgG)。試劑盒中使用的特異性抗體為兔多克隆抗體。免疫原是一種CHO衍生的重組蛋白。標(biāo)準(zhǔn)品為CHO衍生的重組蛋白。對(duì)于犀牛樣品,我們沒有使用牛或馬的ELISA試劑盒進(jìn)行測(cè)試。如果您想試一試,我們建議你先做一個(gè)預(yù)實(shí)驗(yàn)。

靶點(diǎn)詳情

  • 功能:
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接:


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