Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

倉(cāng)鼠脂聯(lián)素，含C1Q和膠原域(ADIPOQ)酶聯(lián)免疫試劑盒（CSB-EL001366HA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ADIPOQ含量。ADIPOQ是脂聯(lián)素基因。脂聯(lián)素由脂肪組織分泌，具有抗糖尿病、抗炎、抗動(dòng)脈粥樣硬化等作用。研究機(jī)制上，它可與多種受體結(jié)合，激活下游信號(hào)通路，調(diào)節(jié)能量代謝、改善胰島素敏感性，在代謝性疾病等研究中受關(guān)注。試劑盒檢測(cè)范圍為0.469 ng/mL-30 ng/mL，該產(chǎn)品適用于科研實(shí)驗(yàn)中評(píng)估倉(cāng)鼠模型在代謝疾病干預(yù)、脂肪組織功能研究、運(yùn)動(dòng)生理學(xué)或藥物開(kāi)發(fā)過(guò)程中ADIPOQ表達(dá)水平的變化，為探索脂聯(lián)素在能量代謝調(diào)控機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

ADIPOQ
Uniprot No.：

C9DFG2
種屬：

Mesocricetus auratus (Golden hamster)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	82-90
1:2000	Average %	93
	Range %	90-96
1:4000	Average %	89
	Range %	84-93
1:8000	Average %	94
	Range %	90-98

回收率：

The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.