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  4. Fish Interleukin 1β (IL- 1β) ELISA Kit

Fish Interleukin 1β (IL- 1β) ELISA Kit

  • 中文名稱：
    魚白介素1β(IL-1β)酶聯免疫試劑盒
  • 貨號：
    CSB-E13259Fh
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    魚白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E13259Fh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B是重要的炎癥細胞因子，在多種炎癥性疾病及免疫反應中起關鍵作用。研究機制上，它參與激活免疫細胞、調節炎癥信號通路。圍繞IL1B的研究聚焦于其在疾病發生發展中的作用，為開發抗炎、免疫調節藥物提供靶點。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本產品廣泛應用于水產動物免疫學研究、魚類病原感染模型構建、疫苗效力評估以及水產養殖環境脅迫對機體免疫功能影響等科研場景，為深入解析魚類先天免疫機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    il1bInterleukin-1 beta ELISA Kit
  • 縮寫：
    IL-1β
  • 種屬：
    Fish
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 85    
    Range % 82-89    
    1:2 Average % 94    
    Range % 90-99    
    1:4 Average % 104    
    Range % 97-108    
    1:8 Average % 99    
    Range % 94-106    
  • 回收率：
    The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-102    
    EDTA plasma (n=4) 89 82-95    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    200 2.989 2.778 2.884 2.776    
    100 2.467 2.432 2.450 2.342    
    50 1.774 1.866 1.820 1.712    
    25 0.956 0.932 0.944 0.836    
    12.5 0.436 0.456 0.446 0.338    
    6.25 0.258 0.270 0.264 0.156    
    3.12 0.156 0.155 0.156 0.048    
    0 0.105 0.111 0.108 　    
  • 數據處理：
  • 貨期：
    3-5 working days

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