Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14055c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP-13含量。MMP-13即基質(zhì)金屬蛋白酶13，在人體組織重塑、炎癥反應(yīng)等生理過程中起重要作用。異常表達(dá)與多種病理情況相關(guān)，如關(guān)節(jié)炎、癌癥等。研究主要圍繞其酶活性調(diào)控機(jī)制，旨在開發(fā)針對(duì)此靶點(diǎn)的藥物，以干預(yù)相關(guān)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域?qū)θ惞顷P(guān)節(jié)疾病模型、軟骨退行性病變機(jī)制以及抗炎藥物研發(fā)等研究方向的樣本檢測(cè)需求；滿足實(shí)驗(yàn)室對(duì)犬MMP - 13蛋白表達(dá)水平的高通量檢測(cè)及動(dòng)態(tài)監(jiān)測(cè)要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP-13
Uniprot No.：

F1PVS9
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	99
	Range %	95-106
1:4	Average %	87
	Range %	84-92
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	88	85-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.