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Bovine Interleukin 5 (IL-5) ELISA Kit

  • 中文名稱:
    牛白介素5(IL-5)酶聯免疫試劑盒
  • 貨號:
    CSB-E12897B
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    7.8 pg/mL-500 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 別名:
    IL5 elisa kit; IL-5 elisa kit; Interleukin-5 elisa kit; Il5 elisa kit
  • 縮寫:
    IL-5
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    86-98

    1:2

    Average %

    99

    Range %

    92-103

    1:4

    Average %

    87

    Range %

    82-94

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of bovine IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-102

    EDTA plasma (n=4)

    97

    94-106

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.596

    2.501

    2.548

    2.490

    250

    1.492

    1.456

    1.474

    1.415

    125

    0.827

    0.845

    0.836

    0.778

    62.5

    0.471

    0.496

    0.484

    0.425

    31.2

    0.272

    0.257

    0.265

    0.206

    15.6

    0.171

    0.178

    0.175

    0.116

    7.8

    0.124

    0.125

    0.124

    0.066

    0

    0.060

    0.057

    0.058

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    IL-5 family
  • 數據庫鏈接:

    KEGG: bta:280825

    STRING: 9913.ENSBTAP00000041739

    UniGene: Bt.5



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